ProQuant™ is precise, but is it accurate as well?
All of our experimental optimisations during the development of the ProQuant™ proteomics platform were aimed at improving the precision of the method. We have already shown the marked effect that our proprietary optimisations have had:
But there’s a difference between precision and accuracy. There’s a good summary on Wikipedia if you want to delve a bit deeper, but put simply…
Precision is a measure of how close measurements are to each other
Accuracy is a measure of how close measurements are to the true value
By being highly precise, ProQuant™ gives you reproducible and reliable measurements of protein and peptide abundances and PTM fractional modifications. But how close are the measurements to the true value. This is where things get difficult of course, because knowing the true value of anything we attempt to measure in science is notoriously difficult. The approach we took was to compare the results from one of our non-hypothesis driven analyses of human serum with the concentrations of those proteins we obtained from the scientific literature.
It’s not a surprise to find that there is an association between these two datasets, but what shows the superior performance of ProQuant™ is how much better the association is with our data compared with two renowned online databases:
a comprehensive absolute protein abundance database maintained by the Bioinformatics / Systems Biology group at the University of Zurich.
a compendium of results from MS proteomics datasets published by the human proteome organisation.
The conclusion from this is clear. Proteomics analysis using ProQuant™ generates data with higher accuracy than the proteomics methods used by PAXdb and PeptideAtlas.